Additional file 1.

Figure S1. Peptide competition assays. Immunofluorescence microscopic analyses show the specificity of the antibodies used in this study. Grey-scale images were acquired using a Leica DM RB transmission light microscope equipped with a Leica DC300F CCD camera; subsequently false colors were assigned to each channel. Green: post-translational histone modifications. DAPI was used for DNA counterstaining (red). (A) H3K9ac antibody (ab); (B) H3K9ac ab competed with the immunizing peptide; (C) H3K14ac ab; (D) H3K14ac ab competed with the immunizing peptide; (E) H3K4me1 ab competed with a peptide trimethylated at K4; (F) H3K4me1 ab competed with the immunizing peptide; (G) H3K4me3 ab competed with a peptide monomethylated at K4; (H) H3K4me3 ab competed with the immunizing peptide; (I) H3K36me3 ab; (J) H3K36me3 ab competed with the immunizing peptide; (K) H3K36ac ab; (L) H3K36ac ab competed with the immunizing peptide; (M) H3K9me3 ab competed with a peptide trimethylated at K27; (N) H3K9me3 ab competed with the immunizing peptide; (O) H3K27me1 ab competed with a peptide trimethylated at K27; (P) H3K27me1 ab competed with the immunizing peptide; (Q) H3K27me3 ab competed with a peptide trimethylated at K9; (R) H3K27me3 ab competed with the immunizing peptide; (S) H3S28p ab; (T) H3S28p ab competed with the immunizing peptide.

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Postberg et al. Epigenetics & Chromatin 2008 1:3   doi:10.1186/1756-8935-1-3