Additional file 2.

Figure S2. (A) Western analyses confirm the occurrence of post-translational histone H3 modifications in micronuclei (m) and macronuclei (M) as well as in anlagen 24 h and 48 h post conjugation. Piwi/mdp1 (Western blot, left) is not detected in vegetative cell shortly before conjugation (0 h). A < 100 kDa protein is detected during macronuclear development (12 h and 24 h post conjugation) using an antibody targeted to a Piwi family protein. Western blot, right: The band detected with the Piwi antibody (lane wt) becomes weaker or disappears completely upon RNAi treatment (lane RNAi) (method described in [27]); α-tubulin is used as a loading control. (B) DNA isolated from micronuclei (2), anlagen (4) or macronuclei (6) was digested with BglII and separated using agarose gel electrophoresis and transferred to a nylon membrane; (1) is a DNA size marker. In Southern analyses using stad5 as a probe prominent signals in micronuclei (3) and anlagen (5), but not in macronuclei (7) are detected.

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Postberg et al. Epigenetics & Chromatin 2008 1:3   doi:10.1186/1756-8935-1-3