|
Overview about all histone modifications and proteins examined and their nuclear localization in vegetative and differentiating cells. |
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| Vegetative cells |
Differentiating cells |
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|
|
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| ++ = high level + = moderate level - = not detected |
Micronuclei |
Macronuclei |
Micronuclei |
Parental macronuclei |
Macronuclear anlagen (a1 to early a2) |
Macronuclear anlagen (a2to a3) |
Macronuclear anlagen (e) |
|
|
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| Histone acetylation |
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| H3K9ac |
- |
++ |
- |
+4 |
++ |
++ |
++ |
| H3K14ac |
- |
++ |
- |
+4 |
++ |
++ |
++ |
| H3K36ac |
- |
- |
- |
- |
- |
++ |
+4 |
| Histone methylation |
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| H3K4me1 |
- |
++ |
- |
+4 |
- |
++ |
++ |
| H3K4me3 |
- |
++ |
- |
+4 |
- |
++ |
++ |
| H3K9me3 |
- |
- |
- |
+5 |
- |
++ |
+4 |
| H3K27me1 |
- |
- |
- (+1) |
- |
- |
++ |
+4 |
| H3K27me3 |
++ |
- |
++ |
- |
- |
++ |
+4 |
| H3K36me3 |
+ |
- |
+(-2) |
- |
- |
+ |
+4 |
| Histone phosphorylation |
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| H3S28p |
++ |
+ |
- |
+4 |
++ |
++ |
++ |
| Chromatin modifying proteins |
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| E(z)/KMT6-like HMT |
- |
- |
- |
- |
- |
++ |
+4 |
| Spdd1p |
- |
- |
- |
+5 |
- |
++ |
+4 |
| RNAi-related machinery |
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| Piwi/mdp1 |
- |
- |
+3 |
++6 |
++ |
+4 |
+4 |
|
1transient accumulation during conjugation 2transient loss during conjugation 3transient accumulation early during conjugation 4signal intensity and frequency continuously decreasing 5modification occasionally observed as dispersed foci-like signals mostly not visible in stack projections 6resrtricted to conjugation and early exconjugant cells with no visible macronuclear anlagen | |||||||
Postberg et al. Epigenetics & Chromatin 2008 1:3 doi:10.1186/1756-8935-1-3 |
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