|
Resolution: standard / high Figure 8.
Expression of genes near telomeres is unaffected by nst mutations. Total RNA was extracted [47] from N3120 (nst+) and N3132 (nst-1RIP1nst-3RIP1nst-5RIP1) and 20 μg was separated by agarose gel, blotted, and probed as described [48]. The 28S rRNA panel shows equivalent loading in both lanes by methylene blue staining
of a blot. Other panels are autoradiograms after probing with the coding region of
the gene closest to telomere IL (NCU010129.3), VIL (NCU07143.3), or VIIL (NCU09306.3).
Smith et al. Epigenetics & Chromatin 2008 1:5 doi:10.1186/1756-8935-1-5 |