Additional file 1:

Figure S1 A. Silver stain of various SAGA/SLiK(SALSA) purifications. Lane 1 marker, Lane 2 Spt8Tap purification, Lane 3 Gcn5TAP;sgf73Δ purification, Lane 4 Gcn5Tap purification, Lane 5 Spt8TAP;sgf73Δ purification, Lane 6 SLiK(SALSA) purification from an Spt7TAP strain, where Spt7 lacks the C-terminus required for Spt8 association, Lane 7 Sgf73TAP purification B. MudPit analysis of Sgf73TAP purification compared to the Gcn5TAP purifications in the presence or absence of SGF73.

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Additional file 2:

Figure S2 Description: Sgf73 provided in trans is able to incorporate into the SAGA/SLiK (SALSA) complex and partially rescue histone deubiquitination activity. A. Silver stain gel showing purification of SAGA/SLiK(SALSA) from yeast expressing SGF73 from a vector under control of it's own promoter (Lane 1). B. MudPit analysis of the rescued complex compared with the complex purified with only an empty vector. Highlighted rows indicate the proteins that are recruited back into the complex after the addition of Sgf73. C.In vitro deubiquitination assay demonstrating that the rescued complex is able to partially rescue the ability to deubiquitinate histone H2B, Lane 1 negative control, Lane 2 Spt8TAP, Lane 3 Gcn5Tap;sgf73Δ + empty vector, Lane 4 Gcn5Tap;sgf73Δ + SGF73. D. Quantification of data obtained in C.

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