Research
The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis
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* Corresponding author: A Francis Stewart stewart@biotec.tu-dresden.de
- † Equal contributors
1 Genomics, BioInnovationsZentrum, Technische Universitaet Dresden, Am Tatzberg, 01307 Dresden, Germany
2 Centre for Regenerative Therapies Dresden, BioInnovationsZentrum, Technische Universitaet Dresden, Am Tatzberg, 01307 Dresden, Germany
3 Monash Institute of Medical Research, Monash University, Melbourne and ARC Centre of Excellence in Biotechnology and Development, Australia
4 Department of Histology and Cell Biology, School of Medicine, Yokohama City University, Yokohama, Japan
5 The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
6 TaconicArtemis Pharmaceuticals GmbH, Neurather Ring, 51063 Cologne, Germany
7 University of Applied Science Gelsenkirchen, Department of Applied Natural Sciences, August-Schmidt-Ring, 45665 Recklinghausen, Germany
Epigenetics & Chromatin 2009, 2:5 doi:10.1186/1756-8935-2-5
Published: 6 April 2009Additional files
Additional File 1:
Expression profiling in embryonic stem cells. The primary list of down-regulated genes from the Affymetrix experiments. The absence of data in the constitutive columns is due to the fact that these experiments were performed with an earlier version of the mouse array that did not have probes for these genes. The top 10 down-regulated genes found in both constitutive and conditional experiments were evaluated by quantitative RT-PCR. The presence of a CpG island at exon 1 of the respective genes is noted in the right hand column.
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Additional File 2:
Comparison of Magoh and Magoh2. (A) The amino acid sequences of Magoh2 and Magoh are aligned. (B) Expression analysis by quantitative RT-PCR was performed for various mouse tissues taken from a 2-month-old male.
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Additional File 3:
Intestinal complications in Rosa26-CreERT2 homozygous mice. (A, B) An intestinal display of a tamoxifen-treated Rosa26-CreERT2/+ heterozygous mouse (A) and of a tamoxifen-treated Rosa26-CreERT2/Rosa26-CreERT2 homozygous mouse (B). Both mice were Mll2 +/+ and were dissected 10 days after the start of tamoxifen administration (five daily doses of 4 mg). In panel (B) the bile duct (arrow) is swollen, the stomach and small intestine is distended. Diarrhea and a rectal prolapse were also evident. (C) Sick Rosa26-CreERT2 homozygous mice had symptoms of dehydration as indicated by weight loss (data not shown) and increased stomach weights compared with controls. (D) Summary of tamoxifen-provoked deaths in various genotypes.
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Additional File 4:
Additional expression data from mll2FC/FC; Rosa26 CreER(T2)/+ testes. (A) As for Figure 7, quantitative RT-PCR of selected genes. (B) Mean Ct values of quantitative RT-PCR for selected genes on five control (wild-type) testes. (C) Results from Affymetrix analysis of control and mutant testes 6 days after the start of tamoxifen treatment. Only the greatest fold changes, either down (at top) or up (at bottom), are shown, along with p-values. The results are the average of two independent experiments.
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