Figure 7.

Expression analysis by quantitative RT-PCR of selected genes in mll2 mutant and control testes. The expression levels from total testes of controls (black) before (-) or 1 week after tamoxifen were compared with mutants (grey) after tamoxifen administration. All times refer to weeks after the start of five daily doses of 4 mg tamoxifen. Busulfan-treated testes were used to generate Sertoli-cell only controls (SCO). All data is based on at least three mice. The mean value ± SEM of relative expression normalized against the endogenous standard genes GAPDH and Rpl19 is shown (* P < 0.05; ** P < 0.02).