Table 1 |
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Oligos used in the assays |
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Gene name |
Oligo name |
Sequence 5'→3' |
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SEPT9 |
Forward |
GATT-X-GTTGTTTATTAGTTATTATGT |
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Reverse |
AAATAATCCCATCCAACTA |
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|
|
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|
Blocker |
GTTATTATGTTGGATTTTGTGGTTAATGTGTAG-C3 |
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|
|
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|
Probe |
FAM-TTAACCGCGAAATCCGAC-BHQ1 |
|
|
|
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|
Oligo 27 |
ttattagttattatgt |
|
|
|
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Oligo 28 |
cggatttcgcggttaacg |
|
|
|
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Oligo 45 |
cgcgttaaccgcgaaatcc |
|
|
|
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|
TMEFF2 |
Forward |
GGTTATTGTTTGGGTTAATAAATG |
|
|
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|
Reverse |
AAAAAAAAAAAACTCCTCTACATAC |
|
|
|
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|
Blocker |
ACATACACCACAAATAAATTACCAAAAACATCAACAA-C3 |
|
|
|
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|
Probe |
FAM-TTCGGACGTCGTTGTTCGG-BHQ1 |
|
|
|
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|
Oligo 30 |
gttaataaatggagtt |
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|
|
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Oligo 44 |
gaacaacgacgtcc |
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|
|
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Oligo 62 |
cgtcgttgttcggt |
|
|
|
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|
X = tetrahydrofuran-type abasic site. |
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Tierling et al. Epigenetics & Chromatin 2010 3:12 doi:10.1186/1756-8935-3-12 |
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