Figure 1.

Common MLL/COMPASS subunits associate with cytokine-inducible genes. (a-c) Several common subunits of MLL/COMPASS associate with CIITA pIV. ChIP assays were carried out in HeLa cells stimulated with IFN-γ for 0 to 18 hours. Lysates were immunoprecipitated with control or endogenous (a) WDR5, (b) Ash2L or (c) RbBP5 antibody. Associated DNA was isolated and analyzed via real-time PCR using primers and probe spanning CIITA pIV. (d-f) Subunits of MLL/COMPASS associate with the MHC-II proximal promoter. ChIP assays were carried out in HeLa cells stimulated with IFN-γ for 0 to 18 hours. Lysates were immunoprecipitated with control or endogenous (d) WDR5, (e) Ash2L or (f) RbBP5 antibody. Associated DNA was isolated and analyzed via real-time PCR using primers and probe spanning the MHC-II proximal promoter. To demonstrate constitutive binding, data are presented as percentage input. IgG isotype control values were 0.001 ± 0.0005 (CIITA pIV) and 0.01 ± 0.007 (MHC-II promoter). Values represent mean ± SEM of (n = 3) independent experiments.