Additional file 1.
Figure S1 - Identification of protein arginine methyltransferase (PRMT)1 as a histone H2A methyltransferase, using nanoscale liquid chromatography and mass spectrometry (MS). The two fractions (numbers 4 and 6) from the heparin column (Figure 1B) containing methyltransferase activity towards histone H2A 4-129 were analysed by MS as described (see Additional file 6, supplementary methods). Only proteins with two unique peptides possessing a total Mascot score of >54 were considered to be significant, and these were used for MS3 scoring in MSQuant software (http://msquant.sourceforge.net webcite). PRMT1 was found in both heparin fractions. IPI and Uniprot accession numbers for PRMT1 are listed.
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Waldmann et al. Epigenetics & Chromatin 2011 4:11 doi:10.1186/1756-8935-4-11