Email updates

Keep up to date with the latest news and content from Epigenetics & Chromatin and BioMed Central.

This article is part of the supplement: Epigenetics and Chromatin: Interactions and processes

Open Access Poster presentation

Elucidating the mechanisms underlying GATA-1 activity

Roland Gamsjaeger1, Ana Silva1, Nicholas Shepherd1, Lorna Wilkinson-White1, Ernest Laue2, Gerd Blobel3, Jacgui Matthews1 and Joel Mackav1*

  • * Corresponding author: Joel Mackav

Author Affiliations

1 School of Molecular Bioscience, University of Sydney, NSW, Australia

2 Department of Biochemistry, University of Cambridge, UK

3 Children’s Hospital of Philadelphia, PA, USA

For all author emails, please log on.

Epigenetics & Chromatin 2013, 6(Suppl 1):P49  doi:10.1186/1756-8935-6-S1-P49

The electronic version of this article is the complete one and can be found online at: http://www.epigeneticsandchromatin.com/content/6/S1/P49


Published:18 March 2013

© 2013 Gamsjaeger et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

The fact that GATA-1 binds to gene regulatory elements that contain GATA sites, and thereby ultimately regulates the expression of hundreds of genes involved in hematopoietic development is well established. However, at a molecular level, the steps between DNA binding and changes in the expression levels of target genes are only partly understood, and the goal of our work is understand at a mechanistic level the changes in chromatin structure and occupancy and the protein interactions that are made to drive these changes in gene expression. We have recently shown that, surprisingly, the localization of GATA1 to its target sites in chromatin is dependent on acetylation of specific lysines in the protein, and have elucidated the structural basis for this requirement [1,2]. Our data point towards a model for gene regulation in which not only are the post-translational modifications of histones essential for establishing gene expression patterns, but equivalent modifications in DNA-binding transcription factors are also an integral part of whatever ‘code’ underlies these patterns.

Concurrently, we have also determined the molecular details underlying the recruitment of the Nucleosome Remodeling and Deacetylase (NuRD) complex to GATA1-dependent gene promoters [3], an event that is essential for normal GATA1 activity. Combined with data that explore the molecular makeup of the NuRD complex, these results provide a glimpse into the mechanisms through which complex coregulator complexes are recruited to target genes and begin to map out the molecular events that drive gene regulation both in erythropoiesis and, by extension, in a range of other tissues.

References

  1. Lamonica , et al.: .

    PNAS 2011, 108:159. Publisher Full Text OpenURL

  2. Gamsjaeger , et al.: .

    Mol Cell Biol 2011, 31:2632. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  3. Lejon , et al.: .

    J Biol Chem 2011, 286:1196. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL